Fluorescence Image Analysis for Quantification of Active Oxygen Induced by Photochemical Reaction
نویسندگان
چکیده
This study was performed to confirm that activated leukocytes are concerned with Vascular Shut Down effect ; VSD in PhotoDynamic Therapy ; PDT. Hydrogen peroxide ;H2O2, which is a kind of active oxygen that came from monocytes, was made visibility under the confocal laser scanning microscope ;CLSM, and tried to quantify the amount of its formation from the fluorescent intensity with image analysis. Fluorescence images acquired by CLSM were analyzed, and the fluorescence intensity was expressed as gray level, which is graded from 0 to 255. Only the fluorescence derived from a monocyte added Zinc coproporphyrin III ; ZnCP-III just before measurement and with HeNe laser irradiation caused fluorescence distribution move to increase as the time course, while no change of distribution was especially observed in other three conditions (only added Zn CP-III, only irradiated with HeNe laser, Non-treated). The result indicates that photochemical reaction occurred due to excitation of photosensitizer, and active oxygen derived from the reaction stimulated monocytes. The activated monocytes, this time for themselves, generated active oxygen and H2O2 was made visibility by dichlorofluorescin ;DCFH fluorescence method. In conclusion, we confirmed that activated monocytes by photochemical reaction are concerned with VSD effect. Keywords— active oxygen, photochemical reaction, vascular shut down, monocyte, confocal laser scannning microscope
منابع مشابه
Compliance of Radiation Dose and Image Quality in a Nigerian Teaching Hospital with the European Guidelines for Pediatric Screen-Film Chest Radiography
Introduction Blue light is a part of the spectrum with the highest energy content, which can reach the retina. The damage that it can cause to the retina is called photochemical or blue-light retinal injury. For the retinal injury assessment of the photochemical and aphakic retinal hazards in the wavelength range of 300-700 nm, use of effective spectral radiance limits (W.m-2.sr-1) seems to be...
متن کاملUV Spectrometric Indirect Analysis of Brominated MWCNTs with UV Active Thiols and an Alkene—Reaction Kinetics, Quantification and Differentiation of Adsorbed Bromine and Oxygen
Indirect UV-absorption spectrometry was shown to be a valuable tool for chemical characterization of functionalized carbon nanotubes (CNTs). It complements data from X-ray photoelectron spectroscopy (XPS) or FTIR analysis since it helps to clarify the type and concentration of functional groups. The principles of indirect application of UV-spectrometry and its mathematical interpretation are di...
متن کاملImpact of Salinity Stress on Photochemical Efficiency of Photosystem II, Chlorophyll Content and Nutrient Elements of Nitere Bush (Nitraria schoberi L.) Plants
Salinity is one of major stresses which can severely limit plant production, especially in the arid and semi-arid regions. The present study was carried out to evaluate the impact of salinity stress on some physio-biochemical parameters in nitre bush plants (Nitraria schoberi). Thus, an experiment was carried out under natural conditions and salinity stress was induced by a combination of diffe...
متن کاملSuperficial radially resolved fluorescence and 3D photochemical time-dependent model for photodynamic therapy.
Photodynamic therapy (PDT) dosimetric tools are crucial for treatment planning and noninvasive monitoring by means of fluorescence. Present approaches consider usually a 1D problem, a simple photochemical process, or a spatially homogeneous photosensitizer. In this work, a radially resolved superficial photosensitizer fluorescence and 3D photochemical time-dependent PDT model are presented. The...
متن کاملAnalysis of estrogenic effects by quantification of green fluorescent protein in juvenile fish of a transgenic medaka.
TheChgH-GFP strain of the teleost medaka contains a regulatory region of the estrogen-responsive choriogenin H (chgH) gene fussed to the green fluorescent protein (GFP) gene. The strain was developed for the identification of environmental estrogens by noninvasive analysis of GFP fluorescence. In the present study, a quantification method for GFP by image analysis was establishedand applied to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2001